BS EN ISO 12966-1:2014:2015 Edition

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Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters – Guidelines on modern gas chromatography of fatty acid methyl esters

Published By	Publication Date	Number of Pages
BSI	2015	18

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Categories: 67.200.10 - Animal and vegetable fats and oils, BSI

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Description

This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs).

The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids of the triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMEs), which are determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation.

The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols, to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards, FAMEs from C4 to C26 can be determined, including saturated fatty acid methyl esters, cis– and trans-monounsaturated fatty acid methyl esters, and cis– and trans-polyunsaturated fatty acid methyl esters.

For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoid interferences with the solvent peak and in order to reduce differences in detector responses.

PDF Catalog

PDF Pages	PDF Title
6	Foreword
7	Introduction
9	Section sec_1 Section sec_2 Section sec_3 1 Scope 2 Normative references 3 Principle
10	Section sec_4 Table tab_1 4 Preparation of FAME
11	Section sec_5 5 Columns
12	Section sec_6 Section sec_7 Section sec_7.1 Section sec_7.2 6 GLC of FAMEs 7 Evaluation of the chromatograms 7.1 Peak area and area per cent 7.2 Evaluation by means of an internal standard or correction factors
13	Section sec_8 8 Test report
14	Reference ref_1 Reference ref_2 Reference ref_3 Reference ref_4 Reference ref_5 Reference ref_6 Reference ref_7 Reference ref_8 Reference ref_9 Reference ref_10 Reference ref_11 Reference ref_12 Reference ref_13 Reference ref_14 Reference ref_15 Reference ref_16 Reference ref_17 Bibliography
15	Reference ref_18 Reference ref_19 Reference ref_20 Reference ref_21

Additional information

Status	Under Review
Title	Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters – Guidelines on modern gas chromatography of fatty acid methyl esters
Corrects	BS EN ISO 12966-1:2014
Replaces	BS EN ISO 15304:2002, BS EN ISO 5508:1995
Publisher	BSI
Committee	AW/307
Pages	18
Publication Date	2015-06-30
ISBN	978 0 580 90475 2
Standard Number	BS EN ISO 12966-1:2014
Identical National Standard Of	ISO 12966-1:2014, EN ISO 12966-1:2014/AC:2015
Descriptors	Chemical analysis and testing, Animal fats, Vegetable oils, Animal oils, Reproducibility, Methyl compounds, Determination of content, Fatty acids, Testing conditions, Oils, Column chromatography, Resolution, Test equipment, Esters (carboxylic), Gas chromatography, Vegetable fats, Chromatography, Error correction, Fats
ICS Codes	67.200.10 - Animal and vegetable fats and oils

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BS EN ISO 12966-1:2014:2015 Edition

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