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BS EN ISO 20976-1:2019

BS EN ISO 20976-1:2019

$167.15

Microbiology of the food chain. Requirements and guidelines for conducting challenge tests of food and feed products – Challenge tests to study growth potential, lag time and maximum growth rate

Published By Publication Date Number of Pages
BSI 2019 38
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This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.

The use of this document can be extended to yeasts that do not form mycelium.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
4.1 General
14 4.2 Estimation of the growth potential
15 4.3 Estimation of the growth kinetics parameters (lag time and maximum growth rate)
5 Apparatus
6 Culture media and reagents
16 7 Study design and sampling
7.1 General
7.2 Setting decision criteria for growth potential
7.3 Number of batches and selection criteria
7.4 Preparation of the test units
17 7.5 Number of test units to be inoculated
8 Selection of strains
18 9 Preparation of the inoculum
9.1 General
9.2 Preparation of the vegetative cell suspensions
9.3 Preparation of the spore suspensions
10 Inoculation of the tests units
19 11 Controls
11.1 Food controls
11.2 Control units
20 12 Storage of the test units
12.1 General
12.2 Estimation of growth potential
12.3 Estimation of growth kinetics parameters (lag time and growth rate)
13 Analysis
21 14 Expression of the results
14.1 General
14.2 Growth potential (Δ)
22 14.3 Growth kinetics parameters (lag time and growth rate)
15 Test report
15.1 General
15.2 Aim of the study and type of challenge test
23 15.3 Experimental protocol
15.4 Sample analysis
15.5 Results
24 15.6 Conclusions
15.7 Reference documents
25 Annex A (informative) Inter-batch variability assessment based on pH and aw
26 Annex B (normative) Minimum number of units to prepare for the challenge test study
27 Annex C (informative) Examples of protocols to prepare inocula
30 Annex D (informative) Examples of how to estimate growth potential, lag time and maximum growth rate from results of challenge tests
34 Annex E (informative) Use of simulation to assess a microbial population under different temperature conditions
35 Bibliography

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BS EN ISO 20976-1:2019
$167.15