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BS EN ISO 21149:2017 – TC:2020 Edition

BS EN ISO 21149:2017 – TC:2020 Edition

$217.84

Tracked Changes. Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria

Published By Publication Date Number of Pages
BSI 2020 71
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PDF Catalog

PDF Pages PDF Title
37 National foreword
39 European foreword
Endorsement notice
42 Foreword
44 1 Scope
2 Normative references
3 Terms and definitions
45 4 Principle
4.1 General
4.2 Plate count
4.3 Membrane filtration
46 4.4 Detection of bacteria by enrichment
5 Diluents, neutralizers and culture media
5.1 General
5.2 Neutralizing diluents and diluents
47 5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution)
5.4 Culture media
50 6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.2.1 General
51 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.3 Counting methods
9.3.1 Dilutions for counting methods
9.3.2 Plate-count methods
52 9.4 Enrichment
9.4.1 General
9.4.2 Incubation of the sample
10 Counting of colonies (plate counts and membrane filtration methods)
11 Detection of growth (enrichment method)
53 12 Expression of results
12.1 Method of calculation for plate count
54 12.2 Interpretation
12.3 Examples
56 12.4 Detection after enrichment
13 Neutralization of the antimicrobial properties of the product
13.1 General
57 13.2 Preparation of inoculum
13.3 Suitability of counting methods
13.3.1 Principle
13.3.2 Suitability test of the pour-plate method
13.3.3 Suitability of the surface spread method
13.3.4 Suitability of the membrane filtration method
58 13.4 Suitability of the detection method by enrichment
13.4.1 Procedure
13.4.2 Interpretation of results
13.5 Interpretation of suitability test results
59 14 Test report
60 Annex A (informative) Other neutralizing diluents
62 Annex B (informative) Other diluents
63 Annex C (informative) Other culture media
66 Annex D (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
67 Bibliography

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BS EN ISO 21149:2017 - TC
$217.84