BS EN ISO 21528-2:2017:2018 Edition
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Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae – Colony-count technique
Published By | Publication Date | Number of Pages |
BSI | 2018 | 24 |
This document specifies a method for the enumeration of Enterobacteriaceae. It is applicable to
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products intended for human consumption and the feeding of animals, and
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environmental samples in the area of primary production, food production and food handling.
This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.
PDF Catalog
PDF Pages | PDF Title |
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2 | undefined |
4 | European foreword Endorsement notice |
6 | Foreword |
7 | Introduction |
8 | 1 Scope 2 Normative references 3 Terms and definitions |
9 | 4 Principle 4.1 Preparation of initial suspension and decimal dilutions 4.2 Isolation and selection for confirmation 4.3 Confirmation 4.4 Calculation 5 Diluent, culture media and reagent 6 Equipment and consumables |
10 | 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 General |
11 | 9.2 Test portion, initial suspension and dilutions 9.3 Inoculation and incubation 9.4 Counting and selection of colonies for confirmation 9.5 Subculturing selected colonies |
12 | 9.6 Biochemical confirmation tests 9.6.1 Oxidase reaction 9.6.2 Fermentation test 9.6.3 Interpretation of biochemical tests 10 Expression of results 11 Performance characteristics of the method 11.1 Interlaboratory study 11.2 Repeatability limit |
13 | 11.3 Reproducibility limit 12 Test report |
14 | 13 Quality assurance |
15 | Annex A (normative) Culture media and reagents |
19 | Annex B (informative) Method validation studies and performance characteristics |
22 | Bibliography |