BSI 20/30360293 DC 2020

This document specifies a procedure for the determination of five Alternaria toxins in wheat, tomato puree and sunflower seeds by high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS).

The method has been validated with both naturally contaminated and spiked samples of wheat, tomato puree and sunflower seeds.

Validation levels for altenuene (ALT) ranged from 2,18 μg/kg to 13,8 μg/kg.

Validation levels for alternariol (AOH) ranged from 1,82 μg/kg to 46,7 μg/kg.

Validation levels for alternariol monomethyl ether (AME) ranged from 1,29 μg/kg to 47,2 μg/kg.

Validation levels for tentoxin (TEN) ranged from 5,29 μg/kg to 218 μg/kg.

Validation levels for tenuazonic acid (TEA) ranged from 41,8 μg/kg to 1 618 μg/kg.

Limits of quantification of 1 μg/kg for ALT (except in wheat and sunflower seeds), AOH and AME; 5 μg/kg for TEN and 10 μg/kg for TEA or lower are achievable using this method.