ISO 3594:1976
$12.35
Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
| Published By | Publication Date | Number of Pages |
| ISO | 1976-12 | 8 |
The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is demonstrated. Peaks of other phytosterols may support this conclusion.
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