ASTM-D6499 2007

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D6499-07 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products

Published By	Publication Date	Number of Pages
ASTM	2007	8

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Category: ASTM

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Description

1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages	PDF Title
1	Scope Referenced Documents Terminology
2	Summary of Test Method Significance and Use Interferences Apparatus Reagents and Materials
3	Hazards Sample Extraction and Preparation Calibration and Standardization Inhibition ELISA Assay Procedure
4	Calculation Report
5	Precision and Bias Keywords TABLE 1 TABLE 2
6	X1. REFERENCE MATERIALS X1.1 X1.2 X2. MODIFICATION OF TEST METHOD D6499 TO INCLUDE PARTICULATES X2.1 Scope X2.2 Sample Extraction and Preparation X2.3 Inhibition Assay Procedure X2.4 Precision and Bias
7	TABLE X2.1
8	REFERENCES TABLE X2.2

Additional information

Standard Title	D6499-07 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products
Published Code	ASTM
Publication Date	2007
Pages Count	8

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ASTM-D6499 2007

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