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ASTM-E1286:2000 Edition

ASTM-E1286:2000 Edition

$35.75

E1286-00 Standard Guide for Identification of Herpes Simplex Virus or its DNA

Published By Publication Date Number of Pages
ASTM 2000 3
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1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of 6.2 and 6.3 of this guide are sufficient.

1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments.

1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpesvirus preparations: this would vary depending on the particular biotechnology use of the virus.

1.4 Warning –Laboratory work involving herpes simplex viruses can be hazardous to personnel. Precaution: Biosafety 2 level facilities are recommended (1) . Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations (1) .

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
Terminology
2 Significance and Use
Background Information About Herpes Simplex Virus
Characterization Criteria for Identification
Report
3 REFERENCES

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