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BS EN 17882:2024

BS EN 17882:2024

$142.49

Food authenticity. DNA barcoding of meat derived from mammals and birds using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments

Published By Publication Date Number of Pages
BSI 2024 24
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This document specifies a method for the identification of meat derived from mammals and birds to the level of genus or species and allows the identification of a large number of commercially important as well as exotic meat species using DNA barcoding. This method was validated on DNA isolated from single pieces of raw meat. This method can also be used for the identification of single meat animal species in some processed products. The described method is unsuitable for the analysis of highly processed foods with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex meat products containing mixtures of two or more meat species. The identification of meat species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI) or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

PDF Catalog

PDF Pages PDF Title
2 undefined
10 8.1 Sample preparation
11 8.2 DNA extraction
8.3 PCR
8.3.1 General
8.3.2 PCR setup
12 8.3.3 PCR controls
8.3.4 Thermal cycling
8.4 Evaluation of PCR products
8.5 Evaluation of the PCR results
13 9.1 Sequencing of PCR products
9.2 Evaluation of sequence data
14 9.3 Comparison of the sequence with public databases
9.3.1 General
9.3.2 Sequence comparison of cytb and/or cox1 amplicon sequences with GenBank®
15 9.3.3 Sequence comparison of cox1 DNA sequences with BOLD

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BS EN 17882:2024
$142.49