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BS EN ISO 10872:2021

BS EN ISO 10872:2021

$142.49

Water and soil quality. Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda)

Published By Publication Date Number of Pages
BSI 2021 32
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This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole freshwater sediment (maximum salinity 5 g/l), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
11 5 Reagents and media
13 6 Apparatus
14 7 Reference substance
15 8 Organisms
8.1 Test organism
8.2 Food organism
9 Stock- and pre‑cultures
9.1 Stock cultures
9.1.1 Caenorhabditis elegans
9.1.2 Escherichia coli
16 9.2 Pre‑culture
10 Procedure
10.1 Preparation of food medium
10.2 Preparation of test material and controls
10.2.1 Soil
17 10.2.2 Sediment
10.2.3 Pore water, elutriate, extract
10.2.4 Solution of reference substance
18 10.3 Test
10.4 Nematode separation
10.5 Measurements and calculations
10.5.1 Recovery
10.5.2 Males
19 10.5.3 Growth and fertility
10.5.4 Reproduction
20 10.6 Timetable of the test
21 11 Validity criteria
12 Expression of results
22 13 Test report
23 Annex A (informative) Determination of maximal water holding capacity (WHCmax)
24 Annex B (informative) Figures and photos of adult worms C. elegans
26 Annex C (informative) Micropipette method (10.3)
27 Annex D (informative) Performance data
30 Bibliography

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BS EN ISO 10872:2021
$142.49