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BSI 22/30432290 DC 2022

BSI 22/30432290 DC 2022

$13.70

BS EN ISO 22174. Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection and quantification of microorganisms. General requirements and definitions

Published By Publication Date Number of Pages
BSI 2022 29
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PDF Catalog

PDF Pages PDF Title
1 30432290-NC.pdf
3 ISO_DIS 22174 ed.2 – id.81777 Enquiry PDF (en).pdf
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
15 4 Principle
4.1 General
16 4.2 Test material
4.3 Sampling, transport and storage
4.4 Preparation of test sample
5 Microbial enrichment and virus concentration
5.1 Microbial enrichment
5.2 Virus concentration
17 6 Nucleic acid preparation
6.1 General
6.2 DNA removal from dead cells
6.3 Nucleic acid extraction, release and purification
6.4 Nucleic acid quality and quantity
18 7 PCR amplification
8 Detection and confirmation of PCR products
19 9 General environmental laboratory requirements
9.1 General
9.2 Laboratory setup
9.2.1 General
20 9.2.2 Control of flows
21 9.2.3 Cleaning of laboratory
9.2.4 Environmental monitoring for PCR
10 Reagents and consumables
22 11 Equipment
11.1 General
11.2 Specific equipment for PCR
11.2.1 Thermal cycler
11.2.2 System for detection of PCR products, comprising
11.2.3 Real-time thermal cycler
11.2.4 Digital PCR thermal cycler (for dPCR)
23 11.2.5 Pipettes (for all PCR formats)
12 Procedure
12.1 Enrichment and sample treatment
12.2 Amplification
12.2.1 General
12.2.2 Control reaction
24 12.2.3 Detection of amplicon
12.2.4 Data analysis
12.3 Evaluation
12.3.1 Qualitative evaluation
25 12.3.2 Quantitative evaluation
26 12.4 Test report
13 Performance characteristics of PCR based methods
14 Validation and verification of PCR based methods
14.1 General
27 14.2 Validation
14.3 Verification
28 Bibliography

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BSI 22/30432290 DC 2022
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