BSI PD ISO/TS 18827:2017
$142.49
Nanotechnologies. Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
| Published By | Publication Date | Number of Pages |
| BSI | 2017 | 32 |
This document provides a procedure for the detection of ROS (OH, O2–,1O2) generated by metal oxide nanomaterials in aqueous solution with a reactive oxygen species-specific spin trapping agent using ESR, but excludes ESR procedures that do not use a spin trapping agent.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 7 | Foreword |
| 8 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms, definitions and abbreviations 3.1 Terms and definitions |
| 10 | 3.2 Abbreviations 4 Principle 4.1 General 4.2 Spin trapping method 4.2.1 General 4.2.2 DMPO |
| 11 | 4.2.3 BMPO 4.2.4 TPC 4.3 Positive control for generating free radicals 4.3.1 Fenton reaction[14] 4.3.2 Hypoxanthine–xanthine oxidase system[15] |
| 12 | 4.3.3 Rose bengal photosensitization[16][17] 5 Reagents 6 Apparatus |
| 13 | 7 Sampling 7.1 Preparation of test sample (metal oxide nanomaterial suspension) 7.2 Preparation of solution for generating the hydroxyl radical 7.2.1 FeSO4 solution 7.2.2 H2O2 solution 7.3 Preparation of solution for generating the superoxide anion radical 7.3.1 Phosphate buffer 7.3.2 Hypoxanthine solution 7.3.3 Xanthine oxidase solution 7.4 Preparation of solution for generating the singlet oxygen |
| 14 | 7.5 Preparation of spin trapping agent 7.5.1 General 7.5.2 DMPO stock solution 7.5.3 BMPO stock solution 7.5.4 TPC stock solution 7.6 Reaction of test sample and spin trapping agent 7.6.1 General 7.6.2 DMPO reaction |
| 15 | 7.6.3 BMPO reaction 7.6.4 TPC reaction 7.7 Reaction of positive control and spin trapping agent 7.7.1 DMPO radical adduct form (DMPO/OH) 7.7.2 BMPO radical adduct form (BMPO/OOH) 7.7.3 TPC radical adduct form (TPC/1O2) 7.8 Preparation of the standard sample for spin calculation |
| 16 | 8 Interferences 8.1 Sampling 8.2 Sampling time 9 Procedure 9.1 General |
| 17 | 9.2 Injection of sample |
| 18 | 9.3 ESR measurement |
| 24 | 10 Examples of results 10.1 DMPO/OH 10.2 BMPO/OOH 10.3 TPC/1O2 |
| 25 | 10.4 TEMPOL |
| 26 | Bibliography |
