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DIN EN 16162:2012 Edition

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Animal feeding stuffs – Determination of decoquinate by HPLC with fluorescence detection

Published By Publication Date Number of Pages
DIN 2012-06 26
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This European Standard specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives. The method was fully validated from LOQ to 60000 mg/kg on different matrices during an international collaborative study, especially on complete compound feeds for poultry, at trace contamination level of 3 mg/kg and at European authorized level of 20 mg/kg to 40 mg/kg. The limit of detection is between 0,1 mg/kg and 0,3 mg/kg and the limit of quantification is around 0,5 mg/kg. These limits were validated during the collaborative study, from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested. This European Standard has been developed to quantify decoquinate in feeding stuffs to enable the European Commission to control the content of animal feed products. However, this method can also be used to evaluate the cross contamination from medicated feed to feedstuff.

DIN EN 16162
$33.80