{"id":340975,"date":"2024-10-19T23:53:53","date_gmt":"2024-10-19T23:53:53","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-102732017\/"},"modified":"2024-10-25T23:04:55","modified_gmt":"2024-10-25T23:04:55","slug":"bs-en-iso-102732017","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-102732017\/","title":{"rendered":"BS EN ISO 10273:2017"},"content":{"rendered":"<p>This document specifies a horizontal method for the detection of <i>Y. enterocolitica<\/i> associated with human disease. It is applicable to<\/p>\n<ul>\n<li>\n<p>products intended for human consumption and the feeding of animals, and<\/p>\n<\/li>\n<li>\n<p>environmental samples in the area of food production and food handling.<\/p>\n<\/li>\n<\/ul>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>2<b><\/td>\n<td>National foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>4<b><\/td>\n<td>European foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>Foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>Introduction  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Terms and definitions  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>4 Abbreviated terms <br \/> 5 Principle <br \/> 5.1 General <br \/> 5.2 Direct plating from liquid enrichment medium <br \/> 5.3 Enrichment in liquid enrichment medium and selective liquid enrichment medium <br \/> 5.4 Plating out after enrichment and identification  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>5.5 Confirmation <br \/> 6 Culture media and reagents <br \/> 7 Equipment and consumables <br \/> 7.8 Peristaltic blender. <br \/> 8 Sampling  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>9 Preparation of test sample <br \/> 10  Procedure (as shown in Annex A) <br \/> 10.1 Test portion and initial suspension <br \/> 10.2 Direct plating on selective agar  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>10.3 Enrichment <br \/> 10.4 Plating out and incubation of plates <br \/> 10.4.1 Plating from PSB and ITC by KOH treatment on CIN agar <br \/> 10.4.2 Plating from PSB and ITC by KOH treatment on chromogenic agar (optional) <br \/> 10.5 Identification of characteristic colonies  <\/td>\n<\/tr>\n<tr>\n<td><b>16<b><\/td>\n<td>10.6 Confirmation <br \/> 10.6.1 General <br \/> 10.6.2 Selection of colonies for confirmation <br \/> 10.6.3 Determination of pathogenic Yersinia species  <\/td>\n<\/tr>\n<tr>\n<td><b>18<b><\/td>\n<td>10.6.4 Confirmation of pathogenic Y. enterocolitica  <\/td>\n<\/tr>\n<tr>\n<td><b>20<b><\/td>\n<td>10.6.5 Interpretation of confirmation tests for Y. enterocolitica <br \/> 10.6.6 Interpretation of confirmation tests for pathogenic Y. enterocolitica <br \/> 10.7 Biotyping of Y. enterocolitica (optional) <br \/> 10.7.1 General  <\/td>\n<\/tr>\n<tr>\n<td><b>21<b><\/td>\n<td>10.7.2 Fermentation of xylose <br \/> 10.7.3 Tween-esterase test <br \/> 10.7.4 Fermentation of salicin (optional) and trehalose <br \/> 10.7.5 Indole formation <br \/> 10.7.6 Interpretation of biotyping tests  <\/td>\n<\/tr>\n<tr>\n<td><b>22<b><\/td>\n<td>11 Expression of results <br \/> 12 Performance characteristics of the method <br \/> 12.1 Interlaboratory study <br \/> 12.2 Sensitivity <br \/> 12.3 Specificity <br \/> 12.4 LOD50  <\/td>\n<\/tr>\n<tr>\n<td><b>23<b><\/td>\n<td>13 Test report <br \/> 14 Quality assurance  <\/td>\n<\/tr>\n<tr>\n<td><b>24<b><\/td>\n<td>Annex A (normative) Diagrams of the procedures  <\/td>\n<\/tr>\n<tr>\n<td><b>27<b><\/td>\n<td>Annex B (normative) Composition and preparation of culture media and reagents  <\/td>\n<\/tr>\n<tr>\n<td><b>42<b><\/td>\n<td>Annex C (informative) Method validation studies and performance characteristics  <\/td>\n<\/tr>\n<tr>\n<td><b>44<b><\/td>\n<td>Annex D (informative) Procedure for cold enrichment  <\/td>\n<\/tr>\n<tr>\n<td><b>49<b><\/td>\n<td>Bibliography  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Microbiology of the food chain. Horizontal method for the detection of pathogenic Yersinia enterocolitica<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2017<\/td>\n<td>52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340977,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340975","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340975","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340977"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340975"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340975"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340975"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}