{"id":418523,"date":"2024-10-20T06:21:26","date_gmt":"2024-10-20T06:21:26","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-23-30451046-dc\/"},"modified":"2024-10-26T11:52:05","modified_gmt":"2024-10-26T11:52:05","slug":"bsi-23-30451046-dc","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-23-30451046-dc\/","title":{"rendered":"BSI 23\/30451046 DC"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
8<\/td>\n4 Principle
4.1 General
5 Acanthamoeba trophozoite disinfecting test method
5.1 Organisms
5.2 Culture media and reagents <\/td>\n<\/tr>\n
9<\/td>\n5.3 Test materials
5.4 Test samples
5.5 Culture maintenance <\/td>\n<\/tr>\n
10<\/td>\n5.6 Growth and harvest of microbial challenge (trophozoite)
5.7 Preparation of Acanthamoeba stock solution
5.8 Stand-alone procedure \u2013 inoculation <\/td>\n<\/tr>\n
11<\/td>\n5.9 Recovery Procedures
5.9.1 Stand-alone procedure \u2013 recovery method one (12 well plate method)
5.9.2 Stand-alone procedure \u2013 recovery method two (96 well plate method) <\/td>\n<\/tr>\n
13<\/td>\n6 Controls
6.1 Inoculum Control <\/td>\n<\/tr>\n
14<\/td>\n6.2 Recovery medium control
7 Performance Criteria <\/td>\n<\/tr>\n
15<\/td>\nAnnex\u20acA (normative) Preparation of Acanthamoeba growth medium (Ac#6) <\/td>\n<\/tr>\n
16<\/td>\nAnnex\u20acB (normative) Preparation of \u00bc Strength Ringer\u2019s Solution <\/td>\n<\/tr>\n
17<\/td>\nAnnex\u20acC (informative) Maintenance of Acanthamoeba trophozoites and preparation for testing <\/td>\n<\/tr>\n
18<\/td>\nAnnex\u20acD (normative) Preparation of Non-Nutrient Agar (NNA) <\/td>\n<\/tr>\n
20<\/td>\nAnnex\u20acE (informative) Preparation of E.coli Suspension <\/td>\n<\/tr>\n
21<\/td>\nAnnex\u20acF (informative) Preparation of Non-Nutrient Agar (NNA) Plates with E.coli <\/td>\n<\/tr>\n
22<\/td>\nAnnex\u20acG (informative) Preparation of Neutraliser Broth for recovery methods <\/td>\n<\/tr>\n
23<\/td>\nAnnex\u20acH (normative) Reed and Muench Computation Method for Calculation of the 50\u20ac% Endpoint Titre <\/td>\n<\/tr>\n
27<\/td>\nAnnex\u20acI (normative) Spearman-Karber Computation Method for Calculation of the 50\u20ac% Endpoint Titre <\/td>\n<\/tr>\n
29<\/td>\nAnnex\u20acJ (normative) Micrographs of Cultures Using the 96 Well Plate Method <\/td>\n<\/tr>\n
33<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products – Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2023<\/td>\n33<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":418532,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-418523","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/418523","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/418532"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=418523"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=418523"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=418523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}